In order to understand the composition of a microbial community, our 16S rDNA- or ITS-based Ampli-Seq and OTU clustering analysis provides taxonomic and diversity profiling for eubacteria or fungi, respectively.
Xenovea offers sequencing service for microbial diversity analyses using the Illumina MiSeq or NextSeq 2000 (2×300 bp) platforms.
Amplicon Sequencing (Ampli-Seq)
Sample: ≥20 µl, ≥1 ng/µl High quality DNA
Library preparation: KAPA HiFi HotStart Ready Mix Kit (Roche) and Nextera XT v2 Index Kit (Illumina)
Inclusions (Sequencing, Bioinformatics, Raw data transfer): Microbial diversity analysis includes sample quality control, library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, and metagenomic report); free data transfer of FastQ files and Reports
Sequencing metrics: Performed on Illumina MiSeq or NextSeq 2000 with 2×300 bp paired-end run; Seq throughput: 0.2M PE read per sample; Q30 ≥ 75%
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: min. 2 – max. 4 weeks
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