WGBS Specifications
Sample: ≥20 μl, ≥100 ng/μl High quality DNA
Library preparation: EZ DNA Methylation Lightning MagPrep Kit (Zymo) and NextFlex Rapid XP DNA-Seq kit v2 with UDIs (PerkinElmer)
Inclusions (Sequencing, Bioinformatics, Raw data transfer): WGBS analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation and variant reports);
free data transfer of FastQ, Bam files and Reports
Sequencing metrics: Performed on Illumina NovaSeq 6000 with 2×150 bp paired-end run; Seq throughput: 120 Gbp per sample; 30x coverage; Q30 ≥ 85%
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: min. 2 – max. 4 weeks
WGEMS Specifications
Sample: ≥20 μl, ≥10 ng/μl High quality DNA
Library preparation: NEBNext Enzymatic Methyl-seq library preparation system from New England Biolabs (NEB)
Inclusions (Sequencing, Bioinformatics, Raw data transfer): WGEMS analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation and variant reports);
free data transfer of FastQ, Bam files and Reports
Sequencing metrics: Performed on Illumina NovaSeq 6000 with 2×150 bp paired-end run; Seq throughput: 120 Gbp per sample; 30x coverage; Q30 ≥ 85%
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: min. 2 – max. 4 weeks
RRBS Specifications
Sample: ≥20 μl, ≥20 ng/μl High quality DNA
Library preparation: Ovation RRBS Methyl-Seq system (NuGEN)
Inclusions (Sequencing, Bioinformatics, Raw data transfer): RRBS analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation and variant reports);
free data transfer of FastQ, Bam files and Reports
Sequencing metrics: Performed on Illumina NovaSeq 6000 with 3 Gbp throughput per sample; Q30 ≥ 85%
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: min. 2 – max. 4 weeks
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