Human Whole Genome Sequencing (WGS)

Sample: ≥20 μl, ≥25 ng/μl High quality DNA

 

Library preparation: NEXTFLEX Rapid XP DNA Seq Kit v2 with UDIs

 

Inclusions (Sequencing, Bioinformatics, Raw data transfer): WGS analysis includes sample quality control, library preparation, final QC, sequencing and optinal full bioinformatic analysis (filtering, trimming, mapping, variant calling, annotation and reports); free data transfer of FastQ, Bam, Vcf files and Reports

 

Sequencing metrics: Performed on Illumina NovaSeq with 2×150 bp paired-end run; Seq throughput: ≥ 30x genome coverage, Q30 ≥ 85%

 

Quality control: Full quality assurance and guaranteed specifications

 

Turnaround time: min. 2 – max. 4 weeks