Sample: Tissue/biosample on dry ice or ≥20 μl, ≥20 ng/μl High quality RNA (RIN>7)
Library preparation: Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with Poly(A) Beads 2.0 (PerkinElmer)
Inclusions: mRNA-Seq analysis includes sample quality control, library preparation, final QC
Sequencing metrics: Performed on Illumina NovaSeq with 2x150 bp paired-end run; Seq throughput: 50/100 M PE read per sample; Q30 ≥ 85%
Basic Bioinfromatics: Filtering, trimming
Raw data transfer: Free data transfer of FastQ files
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: 4-5 weeks
