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mRNA-Seq
Targeting poly(A) RNAs provides a sensitive option for detection and quantitation of even rare coding transcript variants

mRNA-Seq

PriceFrom €125.00

  • Sample: ≥20 μl, ≥20 ng/μl High quality RNA (RIN>7)

     

    Library preparation: Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with Poly(A) Beads 2.0 (PerkinElmer)

     

    Inclusions (Sequencing, Bioinformatics, Raw data transfer): mRNA-Seq analysis includes sample quality control, library preparation, final QC, sequencing and optional full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and reports); free data transfer of FastQ, Bam files and Reports

     

    Sequencing metrics: Performed on Illumina NextSeq/NovaSeq with 2x≥75 bp paired-end run; Seq throughput: 50/100 M PE read per sample; Q30 ≥ 85%

     

    Quality control: Full quality assurance and guaranteed specifications

     

    Turnaround time: min. 2 – max. 4 weeks

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