Sample: Tissue/biosample on dry ice or ≥20 µl, ≥50 ng/µl High quality RNA
Library preparation: Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with RiboNaut rRNA Depletion HMR (PerkinElmer)
Inclusions: RNA-Seq analysis includes sample quality control, library preparation, final QC
Sequencing metrics: Performed on Illumina NovaSeq with 2x150 bp paired-end run; Seq throughput: 50/100/200 M PE read per sample; Q30 ≥ 85%
Basic Bioinformatics: Demultiplexing, filtering, trimming.
Raw data transfer: free data transfer of FastQ files
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: 4-5 weeks
