Gene Expression Profiling (GEx)

Sample: ≥20 μl, ≥20 ng/μl High or Low quality RNA

 

Library preparation: QuantSeq 3’ mRNA-Seq Library Prep Kit (Lexogen)

 

Inclusions (Sequencing, Bioinformatics, Raw data transfer): GEx analysis includes sample quality control, library preparation, final QC, sequencing and optional full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and reports);
free data transfer of FastQ, Bam files and Reports

 

Sequencing metrics: Performed on Illumina NextSeq/NovaSeq with 1x≥50 bp single-end run; Seq throughput: 10/20 M SE read per sample; Q30 ≥ 80%

 

Quality control: Full quality assurance and guaranteed specifications

 

Turnaround time: min. 2 – max. 4 weeks