Sample: Tissue/biosample on dry ice or ≥20 µl, ≥50 ng/µl High or Low quality RNA
Library preparation: QuantSeq 3’ mRNA-Seq Library Prep Kit (Lexogen)
Inclusions: GEx analysis includes RNA quantitation/qualification (QC), library preparation, sequencing
Sequencing metrics: Performed on Illumina NovaSeq with ≥75 bp single-end run; Seq throughput: 10/20 M SE read per sample; Q30 ≥ 85%
Basic bioinformatic analysis: demultiplexing, filtering, trimming
Raw data transfer: free data transfer of FastQ files
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: 4-6 weeks
){kind=link}