Shotgun metagenomic sequencing provides not only taxonomic information, but enables functional analysis of the genes present, providing insight into the important pathways of microbial communities.
Xenovea offers sequencing service for metagenomic analyses using the Illumina NovaSeq platforms.
Shotgun Metagenome Sequencing
Sample: ≥20 μl, ≥10 ng/μl High quality DNA
Library preparation: NextFlex Rapid XP DNA-Seq kit v2 with UDIs (PerkinElmer)
Inclusions (Sequencing, Bioinformatics, Raw data transfer): Our analysis includes sample quality control, library preparation, final QC, sequencing and optional full bioinformatic analysis (filtering, trimming, mapping, taxanomy and functional metagenomic reports); free data transfer of FastQ files and Diversity Report
Sequencing metrics: Performed on Illumina NovaSeq with 2×150 bp paired-end run; Seq throughput: 20/40/60M PE read per sample; Q30 ≥ 85%
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: min. 2 – max. 4 weeks