Shotgun metagenomic sequencing provides not only taxonomic information, but enables functional analysis of the genes present, providing insight into the important pathways of microbial communities.
Xenovea offers sequencing service for metagenomic analyses using the Illumina NovaSeq platforms.
Shotgun Metagenome Sequencing
Sample: biological matrix (e.g. stool, soil) or ≥20 μl, ≥10 ng/μl High quality DNA
Library preparation: NextFlex Rapid XP DNA-Seq kit v2 with UDIs (PerkinElmer)
Inclusions: shotgun Metagenome-Seq analysis includes sample quality control, library preparation, final QC
Sequencing metrics: Performed on Illumina NovaSeq with 2×150 bp paired-end run; Seq throughput: 20/40/60M PE read per sample; Q30 ≥ 85%
Bioinformatics: demultiplexing, trimming, filtering
Raw data transfer: Free data transfer of raw FastQ files
Quality control: Full quality assurance and guaranteed specifications
Turnaround time: 4-5 weeks