Small RNA-Seq

Sample: Tissue/biofluid on dry ice or ≥20 µl, ≥5 ng/µl High quality RNA

 

Library preparation: Nextflex Small RNA-Seq Kit v4 (PerkinElmer)

 

Inclusions: miRNA-Seq analysis includes sample quality control, library  preparation, final QC

 

Sequencing metrics: Performed on Illumina NovaSeq; Seq throughput: 20/40 M PE (10/20 M SE) read per sample; Q30 ≥ 85%

 

Basic Bioinformatics: Demultiplexing, filtering, trimming. 

 

Raw data transfer: free data transfer of FastQ files

 

Quality control: Full quality assurance and guaranteed specifications

 

Turnaround time: 4-6 weeks